Presentation Abstract

Title: P16.072 - Mitochondrial DNA diversity in medieval and modern Romanian population
Keywords: mtDNA; haplogroups; ancient DNA
Authors: R. Cocos1, L. Bohiltea1, S. Schipor2, F. Raicu1,3;
1“Carol Davila”University of Medicine and Pharmacy, Bucharest, Romania, 2" C.I.Parhon" National Institute of Endocrinology, Bucharest, Romania, 3Institute of Anthropology "Francisc I. Rainer", Bucharest, Romania.
Abstract: Ancient DNA population studies may yield interesting results in cases where are indications from archaeology and history that a population demographic modification has taken place and significantly reduce the genetic diversity via bottleneck effect.
We analyzed mtDNA variation using aDNA samples from Piata Universit[[unable to display character: ă]][[unable to display character: ț]]ii archaeological site and modern DNA to study nature and extent of temporal changes in genetic variation in Bucharest region during 16th-19th centuries. The archaeological site of Piata Universit[[unable to display character: ă]][[unable to display character: ț]]ii cemetery unearthed about 676 graves with 900 skeletons. We have analyzed by now 18 aDNA samples out of a total of 150 dental pieces recovered from the skeletons excavated from the cemetery and 74 out of 250 modern DNA samples collected. Standard contamination precautions and authentication criteria were applied. Hypervariable regions I and II of ancient mtDNA were amplified and sequenced using twelve overlapping fragments, each with a length of approximately 100 base pairs. The majority of the ancient and modern mtDNA samples analyzed by now falls into the common West Eurasian mitochondrial haplogroups.
In conclusion, to fully assess the dynamics of the historical population composition by comparing genotypes in a temporal context we have to complete the comparative analysis of all aDNA and modern DNA samples. Moreover, in order to reveal possible genetic data changes caused by a possible population bottleneck corresponding to the waves of lethal epidemics, in which an almost one-third of the population was lost, we will also investigate a set of 17 fast evolving short tandem repeat loci (STR).