Poster Session C Presentations and Light Lunch
Evidence of a new distinct strain of Dengue virus 1 isolated in Key West, Florida
11/14/2012 12:00:00 PM
11/14/2012 1:45:00 PM
, Stephanie L. Richards
, Barry W. Alto
, Chelsea T. Smartt
University of Florida, Vero Beach, FL, United States
Dengue virus (DENV) is a fatal pathogen that is transmitted to humans through the bite of mosquitoes. In November 2010, a dengue outbreak was reported from Monroe County in southern Florida, including ≥ 20 confirmed human cases. The DENV isolated from the human cases in Key West was identified as DENV-1. RNA was extracted from the DENV-1 isolate and was used in RT-PCR reactions to amplify PCR fragments to sequence. Nucleic acid primers were designed to generate overlapping PCR fragments that covered the entire genome. The DENV-1 that was epidemic in Key West has been sequenced for whole genome characterization. The sequence assembly, Genbank searches and recombination analysis have been performed to verify the identity of the genome sequences and to determine percent identity to known DENV-1 sequences. Our studies show that the KW DENV-1 is 99% identical to Nicaragua and Mexico DENV-1 strains. However, the phylogenetic and recombination analysis results suggest that the DENV-1 isolated in Key West is a distinct strain and the KW strain may circulate in Key West, while the results confirm that the KW strain originated from Nicaragua. The local dengue virus circulation may become a threat to public health in Unites States, because domestic tourism can spread the disease more rapidly. Our results suggest that the possibility of dengue virus in Key West merits careful consideration.
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