Presentation Abstract

Program#/Poster#: 436.14/T12
Presentation Title: Antioxidant extracts from rosemary and spearmint improve learning, memory and reduce oxidative stress in samp8 mice
Location: Halls B-H
Presentation time: Monday, Nov 11, 2013, 2:00 PM - 3:00 PM
Topic: ++C.06.c. Oxidative stress
1Geriatrics/GRECC, St. Louis University/VA Med., St Louis, MO; 2Div. of Geriatrics, 3Div. of Geriatrics/Division of Endocrinol., St. Louis Univ. Sch. of Med., St. Louis, MO; 4Res. and Develop., Kemin Foods LC, Des Moines, IA
Abstract: Oxidative damage is considered one of the hallmarks of the aging process. The neuronal dysfunction present in cognitive impairments associated with aging is thought in large part to be from oxidative stress. Both structural and functional damage to mitochondria is present in cognitive disorders, such as Alzheimer’s disease, suggesting that antioxidants that penetrate both the cell and the mitochondria will provide the greatest protection from oxidative stress. The current study was designed to test if two novel, proprietary antioxidant-based ingredients, rosemary extract and spearmint extract standardized to carnosic acid and rosmarinic acid respectively, could improve learning and memory in the SAMP8 mouse model of accelerated aging. We have previously shown the ability of antioxidants to improve learning and memory deficits in the SAMP8 mice and reverse signs of oxidative damage; therefore, the SAMP8 mice are an excellent model to test these antioxidant compounds. SAMP8 mice were placed on one of three extracts. Two rosemary extracts contained carnosic acid (60% carnosic acid and 10% carnosic acid) and one spearmint extract contained 5% rosmarinic acid. Three doses of each extract were tested: 32 mg/kg, 16 mg/kg, 1.6 mg/kg and vehicle control. A 50% SAMP8 backcross strain served as the control which also received vehicle. After 90 days of treatment mice were tested in 3 behavioral tests, T-maze foot shock avoidance, object recognition and lever press. Rosemary extract containing 60% carnosic acid improved acquisition at 32 mg/kg and retention at all three doses in T-maze foot shock avoidance, and was able to improve object recognition and lever press at 16 and 32 mg/kg. Rosemary extract with 10% carnosic acid improved retention in T-maze foot shock avoidance (16 mg/kg) and lever press at 16 and 32 mg/kg. Spearmint with 5% rosmarinic acid improved acquisition (16 and 32 mg/kg) and retention (at all doses) in T-maze. In addition, spearmint with 5% rosmarinic acid improved object recognition at 16 and 32 mg/kg. 4-hydroxynonenal (HNE) was reduced in the brain cortex after treatment with all three extracts compared to the vehicle treated SAMP8. Protein carbonyls were reduced in the hippocampus after administration of rosemary with 10% carnosic acid and spearmint containing 5% rosmarinic acid. The current results indicate that the extracts from spearmint (carnosic acid) and rosemary (rosmarinic acid) have beneficial effects on learning and memory and brain tissue markers of oxidation that occur with age in SAMP8 mice.
Disclosures:   S.A. Farr: A. Employment/Salary (full or part-time):; VA Medical Center. B. Contracted Research/Research Grant (principal investigator for a drug study, collaborator or consultant and pending and current grants). If you are a PI for a drug study, report that research relationship even if those funds come to an institution.; Kemin Foods LC. M.L. Niehoff: None. M.A. Ceddia: None. K.A. Herrilinger: A. Employment/Salary (full or part-time):; Kemin Foods LC. B.J. Lewis: A. Employment/Salary (full or part-time):; Kemin Foods LC. S. Feng: A. Employment/Salary (full or part-time):; Kemin Foods LC. J.E. Morley: None.
Support: Kemin Foods LC

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