Presentation Abstract

Session: 199-Increasing the Awareness of Animals and Environment as Reservoirs for Resistance Genes
Monday, Sep 19, 2011, 1:30 PM - 4:00 PM
Presentation Title: C2-1560 - Seagulls Carrying ESBL-Producing Enterobacteriaceae Along the Shoreline in Miami Beach, Florida
Location: W176c
Presentation Number: C2-1560
Pres. Time: Monday, Sep 19, 2011, 2:00 PM - 2:15 PM
Category: C2
Keywords: ESBL; seagulls; Miami
Author(s): L. Poirel, PhD - Associate Researcher1, P. Nordmann - Professor 1, C. De La Cuesta - Associate Professor 2, T. Cleary - Professor 2, A. Potron - Assistant Professor 1, S. Munoz-Price - Assistant Professor 2;
1Hopital de Bicetre, Le Kremlin Bicetre, France, 2Univ. of Miami, Miami, FL.
Financial Disclosures:  L. Poirel,
Merck Role(s): Research Contractor, Received: Research Grant.
P. Nordmann, None..
C. De La Cuesta, None..
T. Cleary, None..
A. Potron, None..
S. Munoz-Price, None.
Abstract: Background: Extended-spectrum ß-lactamase (ESBL), and in particular CTX-M-producing enterics have become an increasingly common cause of community-acquired infections worldwide. The source of these organisms in the community remains unclear. Recent reports related wild animals and water reservoirs to the spread of CTX-Ms Objective: To determine the degree of enteric colonization with ESBL-producing organisms among seagulls in Miami Beach. Methods: During April 2010, feces from wild seagulls were collected weekly in Miami Beach, Florida. Samples were placed in 5 ml of tryptic soy broth with a vancomycin disc (10 ug/ml) and incubated overnight at 37oC. Using the McFarland turbidity test, any broth with growth was subcultured on ChromID ESBL plates (bioMérieux, France), in order to select for ESBL-producing Gram negatives. After overnight incubation at 37oC, colonies were selected based on morphology and color, and further subcultured for purity. Results: 52 samples were collected, from which 83 enterobacterial and 20 non-enterobacterial isolates were obtained. Nine of the enterobacterial isolates produced an ESBL: 7 E. coli CTX-M producers (5 CTX-M-15, 2 CTX-M-32) and 2 Enterobacter cloacae SHV-7 producers. In addition, 14 E. coli isolates were found to express a plasmidic AmpC, namely CMY-2. Pulsed field gel electrophoresis showed clonal diversity among the ESBL-positive E. coli. By contrast, CMY-2-positive E. coli were monoclonal for the most part. Conclusions: 8% of the enterobacterial isolates recovered from 52 stool samples among Miami Beach seagulls were positive for ESBL production and 17% were AmpC producers. Noteworthy, CTX-M-15 and CMY-2 encoding genes were mainly found, that correspond to the most common ESBL and AmpC determinants identified in human isolates. Wild seagulls could be part of the multiple environmental reservoirs for CTX-M genes in the community, and play a role in the spread of those resistance treats.




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