Presentation Abstract

Program#/Poster#: 719.21/N7
Presentation Title: Sociability and amygdala-dependent memory deficits in Dishevelled mutant mice
Location: Halls B-H
Presentation time: Wednesday, Nov 13, 2013, 8:00 AM - 9:00 AM
Topic: ++C.07.a. Autism: Genetic and animal models
1Dept. of Pediatrics and Inst. of Human Genet., Univ. of California San Francisco, San Francisco, CA; 2Biomed. Resonance Sci. Ctr., Shiga Univ. of Med. Sci., Seta-Tsukinowa, Otsu, Shiga, Japan; 3Dept. of Genet. and Genome Sci., Case Western Reserve Univ., Cleveland, OH
Abstract: Mice with mutations in Dishevelled 1 (Dvl1) were the first mutant mice to display behavioral abnormalities in social domains (Lijam et al. 1997, Long et al., 2004). In mammals, three highly homologous Dishevelled (Dvl) genes have been identified: Dvl1, Dvl2 and Dvl3 (Klingensmith et al., 1996; Sussman et al., 1994; Tsang et al., 1996). Here we evaluate mice heterozygous for Dvl3 (Dvl3+/-), mice with a homozygous knockout of Dvl1 (Dvl1-/-) and mice with mutations in both Dvl1 and Dvl3 (Dvl1 -/-3+/-) to further examine the role of each of these genes in social behavior and related behavioral domains. In addition, Dvl mutant mice expressing Dvl2 transgenes specific to either the Wnt or Wnt/PCP pathway were evaluated and compared on these behavioral tasks to determine the pathway responsible for any phenotypic effects. Dvl3+/-, Dvl1-/- and Dvl 1-/-3+/- mutants all display defective sociability on the three chambered social approach task, measured by time spent sniffing the novel mouse. The wild-type Dvl2 transgene rescued defective sociability in Dvl1-/- and Dvl1-/-3+/- mutants. The PCP-deficient Dvl2 transgene also rescued the defective sociability in the Dvl1-/-3+/- double mutants. In the fear-conditioning task, which assesses amygdala-dependent memory formation, Dvl3+/- mutants did not display any deficits in the amount of time spent freezing in either the contextual or cued condition. Dvl1-/- mutants tended to freeze less than the wild-type controls in contextual fear conditioning. All Dvl1-/- mutants expressing transgenes displayed freezing levels similar to wild-type controls. Dvl1-/-3+/- double mutants also displayed diminished freezing behavior in contextual fear conditioning. This deficit was not rescued by the wild-type Dvl2 transgene. The PCP-deficient Dvl2 transgene, did however, appear to rescue the amygdala-dependent memory deficit in the Dvl1-/-3+/- double mutants. Histological findings suggest that the amygdala of Dvl mutants display increased expression of GABAergic interneurons and abnormalities of dendritic branching. We are currently testing whether these GABAergic and dendritic abnormalities are rescued by any of the transgenes. We expect that these analyses will provide insight into the role(s) of Dvl1 and Dvl3 in social behavior and fear conditioning and the mechanism by which these phenotypic effects occur.
Disclosures:  B.A. Babineau: None. J. Nakatani: None. H. Belinson: None. A. Wynshaw-Boris: None.
Keyword(s): BEHAVIOR
Support: NIH Grant 9R01 NS073159-08
NIH Grant 2T32 GM007085-32

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