Effects of chronic intranasal oxytocin and exposure to novel social partners on expression of c-fos and oxytocin in prairie voles (microtus ochrogaster)
Monday, Nov 11, 2013, 11:00 AM -12:00 PM
++E.03.e. Social behavior
*G. M. DOWNING
, A. M. PERKEYBILE
, J. A. VANWESTERHUYZEN
, C. D. GUOYNES
, C. D. PEIRIS
, C. S. BIONDI
, F. MA
, D. J. PATRON
, T. FONG
, K. L. BALES
Univ. of California, Davis, Davis, CA;
John F. Kennedy High Sch., Sacramento, CA
Oxytocin (OT) is a hormone shown to be involved in social bonding in animal models. Previous studies from our laboratory have shown that chronic administration of low or medium doses of OT (0.08 IU/kg and 0.8 IU/kg) in the prairie vole (
) causes deficits in opposite sex pair bond formation. In the present study, we treated voles with one of three dosages of intranasal OT, or a saline control, from day 21 (weaning) to day 42 (sexual maturity). At day 50, we exposed the voles to one of four different behavioral conditions for one hour: a novel cage, a familiar cage, a novel object, or an opposite sex animal. Following immediately after behavior testing, we collected brains and stored them in a fixative of sucrose and sodium azide. Brains were then sliced at 40 microns on a freezing microtome and stored in cryoprotectant until immunohistochemical procedures could be performed. Slices from the paraventricular nucleus, supraoptic nucleus, medial amygdala, nucleus accumbens and the hippocampus will be examined for expression of c-Fos and oxytocin in the cell bodies. We expect higher co-localization of c-Fos and oxytocin in the high dose and saline-treated males in contrast to those treated with the low or medium doses. We expect to see low and medium dose males to have social deficits in interactions with an opposite sex animal, as well as possible anxious reactions to the novel object.
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