Presentation Abstract

Abstract Number: 1992
Presentation Title: Effects of nickel treatment on H3K4 trimethylation and gene expression
Presentation Time: Monday, Apr 04, 2011, 1:00 PM - 5:00 PM
Location: Exhibit Hall A4-C, Poster Section 3
Poster Section: 3
Poster Board Number: 2
Author Block: Kam-Meng Tchou-Wong, Kathrin Kiok, Zuojian Tang, Thomas Kluz, Adriana Arita, Phillip R. Smith, Stuart Brown, Max Costa. NYU School of Medicine, Tuxedo, NY
Abstract Body: Occupational exposure to nickel (Ni) has been associated with lung cancer. We have previously shown that exposure of the human lung carcinoma A549 cells to nickel for 24 hr significantly increased trimethylated H3K4 (H3K4me3), a transcriptional activating mark that maps to the promoters of transcribed genes. To further understand epigenetic mechanisms underlying nickel carcinogenesis, we performed genome-wide mapping of H3K4me3 by chromatin immunoprecipitation and direct genome sequencing (ChIP-seq) and correlated with transcriptome genome-wide mapping of RNA transcripts by massive parallel sequencing of cDNA (RNA-seq). The effects of nickel treatment on H3K4me3 peaks within 5000 bp of transcription start sites (TSSs) on a set of genes highly induced by nickel in A549 cells and human peripheral blood mononuclear cells were analyzed. This is the first genome-wide mapping of trimethylated H3K4 in the promoter regions of genes induced by nickel exposure which may provide insights into epigenetic mechanism(s) underlying nickel carcinogenesis.