Presentation Abstract

Abstract Number: 5130
Presentation Title: Prostate circulating tumor cells metastasize to bone via E-selectin expressed on endothelial cells
Presentation Time: Wednesday, Apr 10, 2013, 8:00 AM -12:00 PM
Location: Hall A-E, Poster Section 22
Poster Board Number: 29
Author Block: Gunjan Gakhar, Guang YuLee, Marco Seandel, Neil Bander, David Nanus. Weill Cornell Medical College of Cornell Univ., NY, NY
Abstract Body: Abstract
Prostate cancer cells preferentially metastasize to bone. Studies show that the preferential adhesion is due to interactions between prostate tumor cells and E-selectin present on human bone marrow endothelial cells. In our study, we used a parallel flow chamber to identify if prostate circulating tumor cells (CTCs) metastasize using similar interactions. CTCs were investigated based on the fact that majority of prostate tumor cells exhibit prostate specific membrane antigen (PSMA) on their cell surface. Prostate cancer cells, MDA PCa 2b (MDA) were first labeled with anti-PSMA monoclonal antibody J591 conjugated with alexa fluor 488 (J591-488) that recognizes PSMA and is internalized following binding to PSMA. Anti-PSMA labeled MDA cells were then perfused over IL-β stimulated human umbilical vein endothelial cells (HUVECs) using parallel flow chamber under physiological blood flow. Both unlabeled and J591-488 labeled MDA cells showed similar rolling behavior on stimulated HUVECs. This suggests that prostate CTCs can be identified and their interactions with endothelial cells can be studied. Our study will potentially elucidate the mechanism involved in prostate cancer metastasis to bone.