Presentation Abstract

Session Title: Platform: Optical Microscopy and Super Resolution Imaging II
Location: Room 130/131
Presentation Number: 2197-Plat
Presentation Time: 2/18/2014 11:00:00 AM
Abstract Title: THE TOPOLOGICAL ORGANIZATION OF THE INACTIVE X CHROMOSOME IN ITS NATIVE STATE
Author Block: Elizabeth A. Smith1, Gerry McDermott1, Karen Leung2, Barbara Panning2, Carolyn A. Larabell1, Mark A. Le Gros1.
1Anatomy, UCSF, San Francisco, CA, USA, 2Biochemistry and Biophysics, UCSF, San Francisco, CA, USA.
Abstract Body: The three-dimensional topology and compaction profile of an individual chromosome likely influences the expression of its genes. We present the first high-resolution description of the structure of an individual chromosome, the inactive X chromosome (Xi), in its native state. Female mouse pre-B cells were vitrified and then imaged using a pair of emerging imaging techniques, cryogenic confocal fluorescence tomography and soft x-ray tomography (CCFT-SXT). Correlation of these two three-dimensional datasets was performed using fiducial markers visible in both modalities. The Xi was identified in each cell by its strong enrichment in a fluorescently-labeled histone variant, macroH2A-EGFP. We present the overall topology, compaction profile, and volume occupied by the Xi in interphase nuclei. Physical contacts with nucleoli and the nuclear envelope are also described. Examination of eleven individual cells showed that there is considerable variation in the spatial arrangement and compaction profile adopted by the Xi.
Commercial Relationship:  E.A. Smith: None. G. McDermott: None. K. Leung: None. B. Panning: None. C.A. Larabell: None. M.A. Le Gros: None.



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